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pmirglo vectors (wt or mut)  (Promega)

 
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    Promega pmirglo vectors (wt or mut)
    Pmirglo Vectors (Wt Or Mut), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmirglo vectors (wt or mut)/product/Promega
    Average 90 stars, based on 1 article reviews
    pmirglo vectors (wt or mut) - by Bioz Stars, 2026-05
    90/100 stars

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    Sponging relationship between BACE1-AS <t>and</t> <t>miR-6805-5p</t> in HCT-8 cells during C. parvum infection. (A) Subcellular location of BACE1-AS in HCT-8 cells. HCT-8 cells were seeded on a coverslip and were infected with C. parvum for 24 h and then applied for FISH analysis of BACE1-AS using probes conjugated with cy3, with 18S localized in the cytoplasm as a control. The nucleus was visualized by DAPI staining. (B) The mRNA levels of miR-6805-5p in HCT-8 cells transfected with pcDNA3.1(+)-BACE1-AS plasmid or si-BACE1-AS following C. parvum infection at 24 hpi by using RT-qPCR. (C) Putative binding site between BACE1-AS and miR-6805-5p predicted by LncBook. (D) The luciferase activity in HCT-8 cells co-transfected with miR-6805-5p mimics and <t>pmirGLO-BACE1-AS-WT</t> or pmirGLO-BACE1-AS-MUT. Statistical analysis was conducted by using a non-parametric t -test. * P < 0.05, ** P < 0.01. WT, wild type.
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    Image Search Results


    Sponging relationship between BACE1-AS and miR-6805-5p in HCT-8 cells during C. parvum infection. (A) Subcellular location of BACE1-AS in HCT-8 cells. HCT-8 cells were seeded on a coverslip and were infected with C. parvum for 24 h and then applied for FISH analysis of BACE1-AS using probes conjugated with cy3, with 18S localized in the cytoplasm as a control. The nucleus was visualized by DAPI staining. (B) The mRNA levels of miR-6805-5p in HCT-8 cells transfected with pcDNA3.1(+)-BACE1-AS plasmid or si-BACE1-AS following C. parvum infection at 24 hpi by using RT-qPCR. (C) Putative binding site between BACE1-AS and miR-6805-5p predicted by LncBook. (D) The luciferase activity in HCT-8 cells co-transfected with miR-6805-5p mimics and pmirGLO-BACE1-AS-WT or pmirGLO-BACE1-AS-MUT. Statistical analysis was conducted by using a non-parametric t -test. * P < 0.05, ** P < 0.01. WT, wild type.

    Journal: Microbiology Spectrum

    Article Title: LncRNA BACE1-AS delays the propagation of Cryptosporidium parvum through regulating cell apoptosis by targeting the miR-6805-5p/IRF3 axis

    doi: 10.1128/spectrum.02022-24

    Figure Lengend Snippet: Sponging relationship between BACE1-AS and miR-6805-5p in HCT-8 cells during C. parvum infection. (A) Subcellular location of BACE1-AS in HCT-8 cells. HCT-8 cells were seeded on a coverslip and were infected with C. parvum for 24 h and then applied for FISH analysis of BACE1-AS using probes conjugated with cy3, with 18S localized in the cytoplasm as a control. The nucleus was visualized by DAPI staining. (B) The mRNA levels of miR-6805-5p in HCT-8 cells transfected with pcDNA3.1(+)-BACE1-AS plasmid or si-BACE1-AS following C. parvum infection at 24 hpi by using RT-qPCR. (C) Putative binding site between BACE1-AS and miR-6805-5p predicted by LncBook. (D) The luciferase activity in HCT-8 cells co-transfected with miR-6805-5p mimics and pmirGLO-BACE1-AS-WT or pmirGLO-BACE1-AS-MUT. Statistical analysis was conducted by using a non-parametric t -test. * P < 0.05, ** P < 0.01. WT, wild type.

    Article Snippet: In this study, the luciferase reporter plasmids of wild-type BACE1-AS (BACE1-AS -WT), mutant BACE1-AS (BACE1-AS -MUT), wild-type IRF3 ( IRF3 -WT), and mutant IRF3 ( IRF3 -MUT) were constructed by cloning BACE1-AS, the miR-6805-5p binding site-mutated BACE1-AS, 3′ UTR region of IRF3 , and the miR-6805-5p binding site-mutated 3′ UTR region of IRF3 into pmirGLO vectors (Promega, Madison, WI, USA), respectively.

    Techniques: Infection, Control, Staining, Transfection, Plasmid Preparation, Quantitative RT-PCR, Binding Assay, Luciferase, Activity Assay

    Target relationship between miR-6805-5p and IRF3 in HCT-8 cells following C. parvum infection. The mRNA (A) and protein (B) levels of IRF3 in HCT-8 cells transfected with miR-6805-5p mimics or inhibitor following C. parvum infection at 24 hpi by RT-qPCR and Western blot. ImageJ was used to calculate the protein level normalized to GAPDH by densitometry. (C) Putative binding site between the 3′-UTR of IRF3 and miR-6805-5p predicted by miRWalk. (D) The luciferase activity in HCT-8 cells co-transfected with miR-6805-5p mimics and pmirGLO- IRF3 -WT or pmirGLO- IRF3 -MUT. Three independent experiments were performed. Statistical analysis was conducted by using a non-parametric t- test. * P < 0.05.

    Journal: Microbiology Spectrum

    Article Title: LncRNA BACE1-AS delays the propagation of Cryptosporidium parvum through regulating cell apoptosis by targeting the miR-6805-5p/IRF3 axis

    doi: 10.1128/spectrum.02022-24

    Figure Lengend Snippet: Target relationship between miR-6805-5p and IRF3 in HCT-8 cells following C. parvum infection. The mRNA (A) and protein (B) levels of IRF3 in HCT-8 cells transfected with miR-6805-5p mimics or inhibitor following C. parvum infection at 24 hpi by RT-qPCR and Western blot. ImageJ was used to calculate the protein level normalized to GAPDH by densitometry. (C) Putative binding site between the 3′-UTR of IRF3 and miR-6805-5p predicted by miRWalk. (D) The luciferase activity in HCT-8 cells co-transfected with miR-6805-5p mimics and pmirGLO- IRF3 -WT or pmirGLO- IRF3 -MUT. Three independent experiments were performed. Statistical analysis was conducted by using a non-parametric t- test. * P < 0.05.

    Article Snippet: In this study, the luciferase reporter plasmids of wild-type BACE1-AS (BACE1-AS -WT), mutant BACE1-AS (BACE1-AS -MUT), wild-type IRF3 ( IRF3 -WT), and mutant IRF3 ( IRF3 -MUT) were constructed by cloning BACE1-AS, the miR-6805-5p binding site-mutated BACE1-AS, 3′ UTR region of IRF3 , and the miR-6805-5p binding site-mutated 3′ UTR region of IRF3 into pmirGLO vectors (Promega, Madison, WI, USA), respectively.

    Techniques: Infection, Transfection, Quantitative RT-PCR, Western Blot, Binding Assay, Luciferase, Activity Assay